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Antioxidant Activity of Young and Old Namnam (Cynometra cauliflora L.)Leaves Ethanol Extracts

One of the natural defense systems that an organism had to prevent exposure from free radicals is by forming a number of compounds that act as antioxidants. One of the substances that can prevent these free radicals are enzymes produced in the body such as glutathione peroxidase enzymes, catalase, and others. The amount of these antioxidants can be added with intake from outside the body. This antioxidant is a type of natural antioxidant that can be found in plants. Namnam plants contain phytochemical compounds such as flavonoids, tannins, phenolics, and terpenoids. These compounds can act as antioxidants. This study's objective was to use the DPPH method to categorize the antioxidant activity of an ethanol extract of young and old namnam leaves. The extraction procedure used ethanol at a 1:5 ratio through a maceration process for three consecutive days in order to acquire these antioxidants. The samples used were young and old Namnam leaves each for 100 gram. The extract was measurements at a wavelength 517 nm by using an uv-vis spectrophotometer. The method was used DPPH for antioxidant activity. The positive control used was ascorbic acid. After that, the absorbance measurement results obtained will be used to measure the IC50 value. This number represents the antioxidant activity of the extracted substance. Old Namnam leaves generate IC50 results of 9.58 ppm, while young Namnam leaves generate IC50 results of 28.93 ppm. Based on this data, the antioxidants in old Namnam leaves give a greater IC50 value than young Namnam leaves.

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Informasi Detail
Judul Seri
-
No. Panggil
610.5 SYA a
Penerbit
Poltekkes Kemenkes Jakarta II : SANITAS: JURNAL TEKNOLOGI DAN SENI KESEHATAN.,
Deskripsi Fisik
-
Bahasa
English
ISBN/ISSN
2615-8647
Klasifikasi
610.5
Tipe Isi
Teknologi Laboratorium Medis
Tipe Media
Teknologi Laboratorium Medis
Tipe Pembawa
Publish
Edisi
Vol. 14 No. 1. Tahun 2023
Subjek
antioxidant
ethanol extract
Namnam leaves
Info Detail Spesifik
SANITAS: JURNAL TEKNOLOGI DAN SENI KESEHATAN
Pernyataan Tanggungjawab
Versi lain/terkait

Tidak tersedia versi lain

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